ABSTRACT
Pharmaceutical, biological, and environmental sciences require knowledge regarding the physicochemistry of solutes of interest. Most mathematical models used in drug design and pollutant bioaccumulation studies are based on quantitative relationships between physicochemical parameters and solute behavior. The chapter presents four broad classes of physicoehemieal parameters that may be estimated by HPLC techniques: hydrophobic-lipophilic solute parameters, electronic solute parameters, complex formation constants, and miscellaneous parameters. The aqueous solubility of organic solutes can also be related to hydrophobic-lipophilic parameters such as the 1-octanol/water distribution coefficient. The total affinity of HSA for the ligands studied could be evaluated by using mobile phases consisting of a pH 7.4 phosphate buffer with varying concentrations of HSA, the results being in good agreement with values obtained using static methods.
