ABSTRACT
This chapter presents optimal in vitro and animal models for assessing the functions of human activated killer monocytes (AKM). AKM can be monitored preclinically by a wide range of in vitro assays and animal models. As clinical trials with AKM become more widely carried out, we must strive to identify more effective preclinical model systems whenever possible. Before 1980, most research studies focusing on AKM-mediated tumor cell destruction were performed in vitro using murine peritoneal macrophages. Studies performed on the antitumor activities of macrophage precursors are rare. An additional special source for human AKM is found in the tumor specimens themselves. There are many potential problems with the interpretation of growth inhibition assays data; for example, thymidine produced by AKM is an inhibitor of tumor cell H incorporation, and contact inhibition of target cell growth occurs commonly among cells cultured in vitro.
