ABSTRACT
This chapter describes how the approach has been used to improve the expression of cDNA constructs encoding human ax-antitrypsin, human factor and other proteins in the mammary gland, as well as discussing the mechanism that may be involved. A number of strategies have been attempted to improve the expression of cDNA constructs in transgenic mice. The incorporation of homologous introns has been shown to enhance efficiency in a number of cases. Transgene rescue can provide a simple method for enhancing the expression of constructs that are, otherwise, predominantly silent in transgenic mice. Transgene rescue results in the activation of otherwise silent transgenes but the levels of expression obtained are considerably those of the rescuing gene. For many applications this will not matter. This would include targeting the expression of growth factors, receptors and reporter genes where very high levels of expression are not required for efficacy.
