ABSTRACT
The mammary gland has been the focus of intense research over the past decade aimed at the efficient, cost effective production of complex human pharmaceuticals. Genetic stability of a transgene is an important criteria in the maintenance and propagation of a herd for pharmaceutical production. This chapter discusses the production of a glycosylation variant of human tissue plasminogen activator (LAtPA) in the milk of transgenic goats. To test the ability of the goat beta casein promoter to direct high level expression of a heterologous protein to the mammary gland of transgenic goats, the LAtPA cDNA was fused between exons 2 and 7 of the goat beta casein gene. In summary, the data shows that the goat beta casein promoter is capable of directing high level expression of a heterologous protein to the mammary gland of a transgenic goat. The promoter appears unique in its ability to achieve g/L expression levels from a cDNA construct.
