ABSTRACT
Fluorescence in situ hybridization (FISH) on stretched or highly extended chromo-some fibers is known as Fiber-FISH, or molecular combing. While molecular combing has recently been rediscovered and even commercialized, for Fiber-FISH such a renaissance has not happened yet. As Fiber-FISH, also sometimes called nuclear chromatin release, neither needs sophisticated equipment nor is complicated to perform, here the protocol for this approach shall be brought back to molecular cytogeneticists’ minds. The Fiber-FISH approach is a modification from Fidlerová et al., and was previously published elsewhere as a modified version. In case of necessity to study chromosomal changes at a resolution not accessible any more by interphase-FISH and being too large to be visualized in molecular combing, Fiber-FISH is the optimal alternative to correspondingly prepare cytogenetic material.
