ABSTRACT
The method of chromosome microdissection with a subsequent DNA isolation was developed over 40 years ago. It allows physically isolating either a whole or a large fragment of a chromosome. After early works on Drosophila polytene, murine, and human metaphase chromosomes, the method was considerably improved by Senger and colleagues, who first employed a combination of an inverted microscope equipped with a rotating plate, including a deproteinization step, followed by DNA amplification. The development of multiple displacement amplification whole-genome amplification methods makes it possible yet to reduce the amount of starting material and work with a single chromosome copy. Traditionally, preparations for microdissection were dropped on coverslips. The resulted libraries may demonstrate different qualities depending on the repetitive DNA content in studied genomes and chromosomes.
