ABSTRACT
In the postgenomic era, microscopic analysis of human chromosomes is often regarded as an optional procedure in genomic research. Moreover, practical issues in medical genetics cannot be properly addressed without FISH, the essence of which lies in the fluorescence microscopy, visualization approaches, and DNA-based chromosome research. The smallest visible molecular target in the field of the vision determines the microscopic resolution of FISH. The use of specific detection systems or algorithms is the way to increase the microscopic resolution of FISH-based techniques. The essential device for FISH-based microscopic analysis is the fluorescence microscope. The device is required to include a fluorescence light source and a set of fluorescence filters, which are composed of dichroic mirror and excitation and emission filters. Imaging and image analysis are aimed at enhancing the efficiency of FISH.
