ABSTRACT
Application of fluorescence in situ hybridization (FISH) in tissues refers to studies done in interphase cells derived from various kinds of solid tissues from the human body. As in FFPE sections embedded after long fixations times in unbuffered formalin may contain highly degraded DNA not suited for FISH in most clinics now FFPE and cryofixation are done in parallel. Each kind of solid human tissue, which can be either cryo- or FFPE-fixed, is suited for this kind of FISH-study. It is not part of this protocol to describe the production of a cryo- or FFPE-block or how to cut the slices and mount on glass-slides. Even though it has been proven that FISH results from archived, cryo- or FFPE-fixed tissues are at least as reliable, if not even better, when instead of tissue-sections including cut and overlaying nuclei, extracted nuclei are studied, most labs stick to sections.
