ABSTRACT
2Modern preclinical and clinical studies show that the use of diagnostic methods based on the registration of fluorescent radiation can improve the early diagnosis of cancer and other destructive processes of various organs. Currently, many studies are being conducted aimed at studying the processes of oxidative metabolism using methods that register the fluorescence of various fluorophores. New instrument designs and analytical tools for the analysis of diagnostic information are being intensively developed. This chapter provides an overview of the main problems of fluorescence detection methods, experimental studies aimed at studying the effect of the main absorbing tissue chromophores on the fluorescence intensity, examples of applications in various fields of medicine and test objects for device calibration and measurement verification. This chapter does not seek to review all the existing problems of the method and present all possible areas of application, but only focuses on a number of important aspects.
