ABSTRACT

Agricultural production is increasingly dependent on severe weather conditions and the use of germplasm, which is constantly evolving by plant breeders to become permissive to different biotic and abiotic stresses. Therefore, efforts toward the successful crop productivity of stress-tolerant plants are crucial. Nowadays, the tissue culture method of in vitro choice has been studied as a viable potential tool for the development of stress-tolerable plants. Plants’ toleration to both stresses could be identified with empirical selective representative, viz. sodium chloride, polyethylene glycol or mannitol. Only the capable explants survive and are selected to maintain such an environment in the prolonged run. In vitro choice is based upon the onset of genetic variation within cells, tissues and organs during culture and reproduction. The choosing of somaclonal variations appear inwardly rejuvenate plants can remain genetically static and effective within crop enhancement. This chapter discusses the advances in the development of stress-tolerant plants, through in vitro selection-based tissue culture.