ABSTRACT
Several studies have shown that Clinacanthus nutans (CN) extract can inhibit various cell cycle checkpoints and induce apoptosis. Many compounds derived from the plant’s extract are known to have anti-cancer activities. These include phenolic compounds, terpenoids, and sulfur compounds. This chapter aims to provide information about CN’s phytochemicals, anti-cancer activities, and signaling pathways based on experimental design and plant preparation procedures. We discovered that the plant’s anti-cancer capabilities are considerably impacted by its different plant parts. As a result, standardizing the experimental design for studies of the plant is critical to ensuring that research is conducted in a way that minimizes the effects of these procedures. Aside from validating CN’s safety and efficacy, additional research into its in vivo properties can help accelerate the translation of promising preclinical findings into clinical trials. This research should also help to identify CN’s bioactive components and develop new drug candidates for the treatment of different types of cancer.
