ABSTRACT
Biofilm is a syntropic consortium of sessile groups of bacteria which are embedded to the autogenic exopolysaccharide (EPS) matrix. This EPS is responsible for almost 80% of chronic microbial infections. Escherichia coli mediated nosocomial infections in medical devices are mostly known. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR–associated (Cas) proteins are responsible for the adaptive immune system of the various bacteria. The molecular mechanism related to the CRISPR-Cas system has been discovered to meticulously regulate bacterial physiology especially its virulence. This review is mainly focused on the inhibition of the biofilm by targeting the quorum sensing mechanism using CRISPR/Cas. The CRISPR/Cas system mainly targets the autoinducers producing genes in order to abort quorum sensing to inhibit biofilm formation.
