ABSTRACT
Cancer is the second foremost cause of mortality throughout the world and, in search of medicinal alternative, nanoparticles (NPs) with therapeutical utilization are being investigated. To evaluate radical scavenging potential of aluminum oxide (Al2O3) and tin dioxide (SnO2) NPs along with green synthesized silver (Ag) and cupric oxide (CuO) NPs prepared using extracts of Simarouba glauca (SG) and Celastrus paniculatus (CP), we determined their antibacterial activity against Escherichia coli, Streptococcus Pyogenes, and Pseudomonas aeruginosa microbes and also their antioxidant capability by ABTS, DPPH, and nitric oxide inhibition assays. The in vitro anticancer activity was assessed employing MCF-7, HT 29, and MCF-10A cell lines by MTT method, of which the highest inhibition of about 97.24 ± 0.48 µg/mL indicated by CuO-CP NPs on MCF-7 cancer cells was further analyzed for molecular mechanism by cell cycle, apoptosis and caspase 3 expression studies. Later to know their hemato-compatibility with human blood we performed spectroscopic analysis with images captured using SEM and found that they were antihemolytic. Eventually, we conducted the anticancer studies of CuO-CP NPs along with standard using EAC model with C57 mice by noting the changes in growth dimensions, tissue biochemical parameters, hematological profiles, and histopathological examinations.
