ABSTRACT
Loop-mediated isothermal amplification is a highly sensitive, low-cost, single-tube technology to detect the target nucleic acid sequences. LAMP has been widely applied to detect various microbial pathogens. In particular, reverse-transcription LAMP has been used for point-of-care-testing for RNA virus infections. In addition, when conducting clinical tests in large quantities, the number of clinical trials more than doubles, and the advantage of a rapid diagnosis of the LAMP assay may be diluted. Since the multiplex SARS CoV-2 RdRP/N/IC RT-LAMP assay takes less time, compared to the commercial Allplex 2019-nCoV Assay and PowerChek 2019-nCoV Real-time PCR kit, it shows promise for deployment as an on-site molecular diagnostic test.
