ABSTRACT
Pullulanase is a potent enzyme for biotransformation of starch rich agro waste into high end products like high fructose corn syrup and high maltose maple syrup via saccharification process. Although pullulanases have been extensively studied, there are only few available that have industrially desirable characteristics. This has raised a challenge to dig for new sources and/or employ advance engineering approaches. This chapter summarizes the microbial-aided production of pullulanases along with the possible use of waste as a substrate by these microorganisms. The factor that affects the activity of pullulanase and the advancement in the enzyme activity and production scale through use of engineering approaches are discussed. In addition to this the application of pullulanase in starch processing industry, baking industry, brewery industry and in production of cyclodextrin are covered. The challenges that have been a setback for the industrial fulfilment of pullulanases are identified to emphasize the direction of future research.
