ABSTRACT
Next-generation sequencing (NGS) is a flexible set of techniques and approaches used for de novo sequencing of eukaryotic genomes which enables parallel multiplexed analysis of DNA sequences on a massive scale. NGS is timesaving and generates huge amounts of sequencing data at incredibly low cost. NGS is increasingly being explored in development of genetically modified plants that include finding novel genes, disease-resistant genes and better understanding plant–host interaction, metabolome study and effect of environmental conditions on plants. It also helps in easing and speeding up pathogen and metagenome identification. With the advent of omics technology, scientists have been able to discover the genetic makeup of disease-causing pathogens, as well as provide crucial clues to monitor plant–pathogen interaction for crop improvement. NGS and metabolomics have been utilized to determine the host genetic factors that the disease-causing agent engineers during attack or infection, as well as the ability to resistance via reprogramming genetic makeup. This chapter discusses the different NGS technologies available and their possible applications in plant pathology.
