To protect our precious life in the ocean of diseases, new materials and applications are utmost important. In this chapter, various green synthesis methods of nanomaterials have been discussed. The nanomaterials have been synthesized taking the advantages of green Chemistry principles, and limitations of nanomaterials are reviewed with profound intensity and depth. The foremost significance of the green synthesis is nonexistence of harmful and deadly toxic chemicals during the synthesis, which helps in protecting human body and at-large environment. Various applications in the health and biomedical sciences have been discussed in this chapter. The chapter discusses about the toxicity generated using nanomaterials and highlights greener pathways as the potential solution. It also covers the applications of biosynthesized nanomaterials in drug delivery, cancer treatment, and other biomedical frontiers.