ABSTRACT
Obesity induced by a high-fat diet was often associated with liver and kidney injury. In this study, GC-FID was adopted to detect the changes in fatty acid content in the liver and kidney of obese mice induced by the high-fat diet. By histopathological examination, obese mice presented a typical characterization of hepatocyte steatosis. Fatty acid detection results showed that the content of total fatty acids in the liver increased in the obesity group, and the metabolic flow of saturated fatty acids was concentrated on C18:0 and its metabolic derivatives. Meanwhile, both n6 and n3 polyunsaturated fatty acids increased significantly in obese mice. In the pathological sections of the kidneys of the obesity group, inflammatory cell infiltration was observed near the glomerulus, which might be an early symptom of obesity-induced liver damage. At the same time, the content of n3 polyunsaturated fatty acids in the kidneys of the obesity group was significantly reduced, and the n6/n3 ratio was also changed. These results were helpful to further understanding the mechanism of liver and kidney injury in obesity and provide a reference for the research and development of weight-loss drugs.
