ABSTRACT
DNA sequencing technologies have shed the light of success to all the current-era discoveries of biological science to achieve a tremendous height. Nanopore sequencing is the most recent in the queue of DNA sequencing techniques. This approach reliably senses the nucleotides with no active DNA production. The nanopore DNA sequencing principles rely on nucleotide-detecting mechanisms. The standard protocol of DNA extraction for nanopore sequencing is always one of the critical steps to achieve success from the procedure. Biological nanopore and solid-state nanopore are the two primary groups of nanopore technologies along with the most recently developed hybrid nanopores. Oxford Nanopore Technologies has 256been constructing nanopore-based systems to sequence DNA for commercial utilization. Several plant species have been used for the Oxford Nanopore Sequencing. Several sensing methods have been suggested and discovered with a great success rate in the last few years, which are strong determining factors for a successful DNA nanopore sequencing method. When a DNA strand translocates through the nanopore, speed control is a major challenge for this DNA sequencing approach. It is being handled with great innovative researches nowadays. The major constraint of the nanopore sequencer is the relatively low reading precision. Many bioinformatics tools have already been released, and several further are in progress, focused, or optimized for nanopore sequencing.
