ABSTRACT
Agri-industrial biomass-derived macromolecules represent a rich source of value-added products. It is vital to bioprocess biomass in an economical way. Enzymatic bioprocessing is preferred to valorize the biomass. The low efficiency, stability, and activity of enzymes are the main obstacles in bioprocessing due to the recalcitrance of agri-based biomass. Protein engineering is one of the potential approaches that has been employed for improving the stability and catalytic performance of enzymes for agri-industrial biomass processing. This chapter elaborates on the enzymes of biomass processing significance, e.g., cellulases, xylanases, beta-xylosidase, pectinases, mannanases, and various approaches of protein engineering, including directed evolution, random mutagenesis, and site-directed mutagenesis. The promising approaches of protein engineering followed by researchers to improve the physico-kinetic and catalytic properties of biomass hydrolyzing enzymes are discussed.
