ABSTRACT
In this study the 65% hydro alcoholic extracts of Holarrhena antidysenterica L. Leaves + Bark (Apocynaceae) affects experimental animals’ toxicity acute and sub- acute. In mice, acute toxicity was investigated with a limit dose of 2000 milligram/kg. For 22–24 hours, observations were made and recorded, followed by 14 days of once-daily observations, and numerous observations such as mortality, behaviour, injuries, and any signs of disease were made. For the sub-acute trial, four groups of six animals (3 males and 3 females) were given 10% Polysorbate 20 in pure water Ctrl, as well as (250, 500, and 1000) milligram/kg of anew formed extract, 24 hr each one orally for days 28. Hematologic and biological investigations were assessed at the conclusion of each research. The livers were dissection for gross abnormalities and compared to the controls group I. In the relative organs, body weights, haematological, biochemical parameters, and gross deformity, there was no significant difference in acute and sub-acute toxicity (p>0.05) in-comparison to control. There none of the deaths rate was recorded. As a consequence of the findings, it is possible to conclude that medium-term oral ingestion of HAE (leaves+ Bark) for days 28 wouldn’t produce lethality.
