ABSTRACT
To demonstrate the effect of oils extracts that return to three plants rosemary (R. officinalis), moringa (M. oleifera) and neem (A. indica) in inhibiting the growth of eight pathogenic isolates, represent in S. typhi, S. aureus, S. dysanteriae, K. pneumonia, P. aeroginosa E. coli, C. lipolytica, B. subtillus. Diffusion in agar gel method (In vitro) was conduct to investigate the effects of the oils extracts in preventing the growth of microorganisms at four concentrations (250–500–750–1000) ppm. The results showed that all oil extracts of the three plants exhibited anti- biologic activity against isolates for all concentrations. Rosemary achieved the superiority results at a concentration (1000) ppm with inhibition diameters (24,21,20,25,23,22,23,21) mm against (S. typhi, S. aureus, S. dysentariae, K. pnumoniae, P. aeroginosa, E. coli, C. lipolytica and B. subtiltus) respectively, while the neem achieved the lowest average (12, 13, 11, 12, 12, 13, 11, 14) mm at concentration (250) ppm. The results also showed that K. pnumoniae and S. typhi were more sensitive to plants oil extracts, while S. aureus and E. coli were more resistance. This study supports the possibility of using rosemary, moringa and neem oil extracts as antimicrobial agents as safe, effective natural source, an alternative to antibiotics, which have negative side effects.
