ABSTRACT
The storage conditions of the soil samples before analysis could have a significant impact on the findings when tracking microbial biomass and activity in soil. Freshly obtained soil is recommended for studies; however, this is not always practicable for practical reasons since sampling is frequently limited to brief seasons of the year, for example because of weather circumstances. Refrigeration and freezing of moist field soil are the two most often utilized techniques for storing soils for microbiological analysis. However, some research have issued warnings against all forms of storage, while other investigations have found no negative effects. This review examined microbial activity, enzyme activities, and community composition while taking into account the long-term consequences of assisted phytostabilization of trace elements. Therefore, a variety of grass species can be useful for the phytoextraction of slightly polluted agricultural soils or the phytostabilization of soils with low-to-moderate concentrations of trace elements, whereas only a few species are suitable for the phytofiltration of trace elements. Utilizing fertilizers and microorganisms that promote plant growth can increase the effectiveness of grasses for phytoextraction or phytostabilization, but the use of such tactics should be carefully considered in each individual situation. Due to the specificity of the trace elements, amendment/microorganism interactions, and plant interactions, increases in the uptake of trace elements by plants can happen instead of greater trace element phytostabilization, and vice versa.
