ABSTRACT
Molecular biology techniques involve the synthesis of cDNA as a central component. In vitro synthesis of cDNA is enzymatically performed, one strand at a time, using RNA as a template by reverse transcriptase (RT). cDNA targets are amplified exponentially through a polymerase chain reaction, which is accomplished using a process known as thermal cycling. This involves repeatedly heating and cooling a solution that contains DNA in order to melt the DNA, anneal short DNA fragments called primers to complementary DNA targets, and enzymatically replicate the primer-bound sequences.
