ABSTRACT
The western blotting technique involves separating proteins by gel electrophoresis, blotting or transferring them onto a membrane, then detecting immobilized antigens selectively. It relies on antibody–antigen interactions and is useful for qualitative or semi-quantitative identification of specific proteins and their molecular weights in complex mixtures. In this chapter, we will describe the required steps for transferring protein from gels to membranes, as well as immunodetection of antigens on a target.
