ABSTRACT
Caries can be caused by Streptococcus mutans, if left untreated, the damage can continue to reach the root canal. Enterococcus faecalis can cause failure in root canal treatment. S. mutans and E. faecalis can grow as biofilms so that they are resistant to antimicrobials. Avocado (Persea americana) seeds contain alkaloids, flavonoids, tannins, steroids, and terpenoids that have antibacterial activity. The purpose of this research to determine the antibiofilm effect of P. americana seeds ethanol extract on the adhesion of S. mutans and E. faecalis biofilms. Fifteen extracted mandibular premolars were cut in the cervical region; the premolar crown then coated with artificial saliva, while the root canal was prepared by biomechanical preparation. Afterward, inoculate S. mutans and E. faecalis for 24 hours. The ethanol extract of P. americana seeds was diluted into 3 concentrations: 25%, 12.5%, and 6.25% with 0.1 NaOH (negative control). The positive control used for S. mutans was 0.2% chlorhexidine (CHX) and 5.25% NaOCl for E. faecalis. The crowns were then soaked with the respective group solution for 30 minutes. The root canals were irrigated with the solution for 10 minutes. Bacterial colonies were then counted using the RT-PCR and Plate Count. Ethanol extract of P. americana seeds could reduce the adhesion of S. mutans and E. faecalis biofilms when compared to the negative control (p<0.05). The best concentration to reduce S. mutans and E. faecalis biofilms was 25%. The ethanol extract of P. americana seeds can inhibit S. mutans and E. faecalis biofilms. Further research is needed.
