ABSTRACT
Background: One of the major issues with dental and oral health is oral cancer. One often used natural component, garlic (Allium sativum), has a wide range of pharmacological effects, including anticancer activity. A. sativum's organosulfur compounds have the ability to hinder the development of cancer cells. Objectives: This research sought to determine the cytotoxicity activity of aqueous and ethanol extracts of A. sativum toward the HSC-3 cell line. Methods: A. sativum extract was applied to HSC-3 cells at doses of 50 g/mL, 100 g/mL, 200 g/mL, 400 g/mL, and 800 g/mL for 24 hours before cytotoxicity test using the MTT method and data analysis using one-way ANOVA and Post-Hoc test (Tukey HSD). Results: Cytotoxicity of aqueous and ethanol extracts of A. sativum varied. Aqueous extract of A. sativum was recognized to be more capable of reducing cell viability at a concentration of 400 µg/mL with a cell viability percentage of 40.229% ± 5.367% and 800 µg/mL with a percentage of 34.482% ± 3.448%. The ethanol extract of A. sativum with a concentration of 800 µg/mL had a cell viability percentage of 40.229% ± 3.448%. Conclusion: In comparison to the ethanolic extract of A. sativum, the aqueous extract significantly reduced the vitality of HSC-3 cells.
