ABSTRACT
To elucidate the effect of oxidative stress on fibroblast cells, it is crucial to create an oxidative stress model in research in vitro as close to the natural pathologic condition. The objective of this study is to evaluate compounds used as oxidative stress inducers on fibroblast cells, the concentrations used, and the signaling pathways investigated. This research's systematic review method was based on the PRISMA guidelines and used the data basis on PubMed publications from 2020 to 2022. There were 431 records identified. Postscreening, there were 179 records left, and 81 passed as eligible, then after the final screening, there were 30 articles included in this research, and from each of the article, information about compound names, compound concentrations used, and pathways that were investigated were collected. From 30 records, hydrogen peroxide (H2O2) was the most used compound to induce oxidative stress, followed by type B ultraviolet (UVB), type A ultraviolet (UVA), and tumor necrosis factor-alpha (TNF-α). The most used concentration for H2O2 was at 100–200 µM, meanwhile for UVB, intensity was at 10–1000 mJ/cm2. The most used signaling pathway in the records was the nuclear factor kappa-light-chain-enhancer of activated B cells (NFkB), followed by mitogen-activated protein kinase (MAPK) and nuclear factor erythroid 2–related factor 2 (Nrf2). The compounds, concentrations, and pathways that were used/investigated on each of the records differ depending on the study's design based on the study's objective created by the respective researchers.
