ABSTRACT
Arumanis mango leaves (Mangifera indica L.) have the potential to be antibacterial and antibiofilm agents as they contain mangiferin, flavonoid, and tannin that might inhibit the growth of P. gingivalis and its biofilm formation. The objective of this study is to determine the antibacterial and antibiofilm effects of Mangifera indica L. leaves ethanol extract against P. gingivalis. An in-vitro laboratory experiment was performed with a post-test only control group design. The present study used 3.125%, 6.25%, 12.5%, 25%, 50%, and 100% concentrations of Mangifera indica L ethanol extract; dimethyl sulfoxide (DMSO) was used as negative control and amoxicillin as positive control. The plate count method was performed for the antibacterial test and microtiter plate biofilm assay for the antibiofilm test. One-way ANOVA was used for the statistical analysis, with p< 0.05 considered as significant level. The result showed the most effective antibacterial activity against P. gingivalis was 100% extract concentration compared to negative control (p<0.05). Moreover, the most effective concentration against P. gingivalis biofilm formation was 100% extract in 3 hours incubation period compared to negative control (p<0.05). It can be concluded that Mangifera indica L. leaves ethanol extract inhibited P. gingivalis growth and biofilm formation.
