ABSTRACT
Foodborne pathogens present major challenges to food safety. Foodborne diseases are one of the most prevailing health issues in the world with interference in the health of the population and the development of society [68]. In the food industry, to ensure microbial food safety, hazard analysis and critical control points (HACCP) and good hygiene practices (GHP) are implemented as a preventive strategy [12]. However, the function of pathogen detection is vital, as it is the key to the identification and prevention of problems. It is essential to control them and for that, early or rapid detection is very crucial [64]. Nucleic acid-based detection assays have become popular and one of the most regularly used platforms to routinely detect foodborne pathogens. Although PCR dominates molecular diagnostics, isothermal amplification methods are gaining momentum recently. There are a number of isothermal amplification methods deployed for the detection of foodborne pathogens. In this chapter, special attention has been given to loop-mediated isothermal amplification (LAMP), rolling circle amplification (RCA), saltatory rolling circle amplification (SRCA), nucleic acid sequence-based 228amplification (NASBA), single primer isothermal amplification (SPIA), recombinase polymerase amplification (RPA), polymerase spiral reaction (PSR), helicase dependent amplification (HDA), cross-priming amplification (CPA), competitive annealing mediated isothermal amplification (CAMP), strand displacement amplification (SDA), and strand exchange amplification (SEA). A detailed appraisal of all the above techniques has been made taking into account the complexity of the assay, instrumentation/reagent necessities, detection limit/time, culture independence, repeatability, portability, etc.
