ABSTRACT
The idea of using whole-cells as catalysts is intriguing, challenging, and a complex task. While considering a particular production process involving the use of a cellular system as a catalyst requires multifactorial adjustments. Developing a whole-cell biocatalyst (WCB) requires a robust chassis with the desired qualities of harboring the natural or acquired enzyme/cascades involved in the production strategies. Not only the physiological adjustments but also the genetic and biochemical adjustments are critical. The availability of information regarding genome annotation and tools for genome editing accelerated the developments in the field of WCB. Efforts of rational protein engineering, developments in the vectors, chimeric nucleases, etc., supported with the in-silico tools for predicting the phenotypes in advance, have laid the support system for designing a robust WCB process of targeted production. Enriched knowledge of modern developed tools for genetic manipulation has aided the development of robust WCB chassis. The plethora of these tools and methods for the advancements in the WCB field has been elaborated in this chapter to help the fraternity for better comprehension of the field.
