ABSTRACT
Biomolecule production by using microorganisms is a common phenomenon that requires the involvement of technical support like scaling up, production strategies, and consumption of enormous quantities of chemicals. Many systems-derived bioengineering tools are involved in biomolecule production in food, medicine, pharmaceuticals, and energy sectors. The systembased tools involved in the molecular simulation, finding enzyme production 174pathways, microbial gene expression modes, biosensor production, and de novo design for microbial production. The expansion of these microbial whole-cell factories is observed in food industries, pharmaceutical sectors, production of industrial chemicals, polymers, therapeutic agents, biocatalysts, amino acids, value-added proteins, biologically important molecules, biofuels, and organic acids. As a result of gene sequencing programs and genomic techniques, the availability of microbial genomes envisages the way to study microbial physiology, molecular mechanics, and metabolic pathways in a lucid manner. The improvement in modern molecular-based techniques provided more efficient microbial whole-cell factories by identifying open reading frames in the gene sequences. Highly defined 3D printing techniques are used to visualize the miniature of bioreactors. This chapter imparts the area of system-based robust microbiological methods like multiomics approach, microbial genome-based modeling; bioengineering enabled tools for the construction of microbial whole-cell factory.
