ABSTRACT
Nanoscience is an approach to generate polymer-based nanocomposite dye for PDT or photodynamic therapy. Polymer molecules can be in preference, retained in tumor, and prevent photosensitizer aggregation. Moreover, stimuli-sensitive polymers can be used for controlled drug release.
Poly(N-isopropylacrylamide) is extensively studied as a promising nano-carrier for biomedical application because of its conformational transition within the physiological temperature. Star-like shape of dextran-graft-poly-N- isopropylacrylamide (D-PNIPAM) used for the creation of multicomponent nanosystem D-PNIPAM/AuNPs/Ce6 nanosystems. These nanosystems were tested by optical absorption in water and in Hank’s buffer solution within a temperature range of 25–40C and by dynamic-like scattering at 25 and 37C. It has been proved that no drastic changes are accompanied by an aggregation process in the nanosystem in Hank’s saline solution in comparison with one prepared in water.
In-vitro examination of the PDT activity of the D-PNIPAM/AuNPs/Ce6 nanosystem on the culture of MT-4 human malignant lymphocytes revealed the absence of cytotoxicity without red-light irradiation. The death of lymphocytes pre-incubated with nanocomposite after laser irradiation was around of 40%.
