ABSTRACT
The importance of trace metal ions in the stimulation and maintenance of growth of mammal cells cultured in vitro has been demonstrated with Zn, Se, Cu, and Va, which increase clonal growth in some cell types. The aim of this study was to evaluate the effect of Al on DNA synthesis and cellular division in human fibroblasts cultured in vitro, using high and low concentrations of Al and different periods of incubation. D Fibroblast cultures used for cell-count experiments were managed basically in the same way as those described above, and were carried out parallel to H-thymidine incorporation. The addition of increasing amounts of Al to the culture medium produces greater DNA synthesis stimulation, which also increases significantly when incubation periods are prolonged from 1 to 2,4, and 5 days.
