ABSTRACT
Integration of bioengineering with additive manufacturing (AM) technology promises to improve the process of generating in vitro models for medical implant applications. As a result, the study of cell–material interactions in 3D printing plays a vital role. Even though AM process has become more common in biological contexts because of its capacity to quickly 3D-print customized physiologically responsive components, limited study has been conducted on cells interacting with 3D-printed material. Specific AM techniques and polymer selection are needed depending on the biological application and complexity of design elements. It is possible by expanding the existing restricted functional 3D-printed biomaterials. Experimental evidence suggests that the 3D-printed scaffold extracts did not cause substantial cell death during in vitro studies. Results also indicated that degraded 3D-printed scaffolds maintained mechanical stability. 3D-printed scaffolds are suitable for bone tissue engineering through cytotoxicity and antimicrobial studies. This current work aims to correlate the cytotoxicity and cell viability of the 3D-printed materials with an interaction with the various cell lines. In the field of bone regeneration, 3D printing of scaffolds has emerged as one of the most cutting-edge surgical procedures to give tailored treatment options with biocompatible implants.
