ABSTRACT
Cryopreservation permits long-term storage of primary cells and cell lines for future use. The cryopreservation process must achieve >90% viability upon defrosting. Furthermore, it is important that the method is performed in a manner that does not cause the cells to burst during the process. This method provides instructions for freezing mesenchymal stem cells (MSCs) from various tissue sources, with notes for adjusting the method to other stem cell types. The described method for MSCs is similar for other stem cells except for mobilized peripheral blood stem cells, which is described in this volume.
