ABSTRACT
Preeclampsia (PE) is a pregnancy-associated disorder that is typically diagnosed by gestational hypertension, proteinuria, or end organ failure. Resident placental stem cells, resembling other tissue-derived mesenchymal stem cells (MSCs), are at the interface of the mother and fetus. The placental stem cells (P-MSCs) can have key roles in proper placentation. These roles can be affected during PE pathogenesis. Thus, it is critical to be able to expand P-MSCs from different regions of the placenta since this will allow for proper dissection of pathologies linked to pregnancy, such as PE. Standardization of the method is critical for scientists across the globe to compare their research studies for proper development of translational studies, including clinical trials. Here, we describe the method to isolate P-MSCs from different placental regions and describe the cell’s characterization by phenotype and functional differentiation.
