ABSTRACT
Supercritical fluid chromatography (SFC) offers big advantages in operating expenses, the rate at which work is accomplished and contributions to the business are made, and environmental friendliness, and is applicable to most separation problems. Relative retention in chromatography is determined by a balance of intermolecular forces acting on the solutes to distribute them between the stationary phase and the mobile phase. The usual pump arrangement for SFC is a binary flow-control pump system with high-pressure mixing. Some means of controlling the column outlet pressure is required in SFC, and this is usually done using a back-pressure regulator. Chiral separations are extremely important in understanding biological systems and in developing agents that interact with or inhibit these systems. Similarly in some ways to the direct fluid introduction interface described earlier for low-flow SFC/mass spectrometric, one may directly introduce the SFC effluent to the atmospheric pressure ionization interface without a post-column pressure-regulation device.
