ABSTRACT
The use of agrochemicals such as pesticides, plant growth regulators, etc., in commercial agriculture has an increasing trend along with the rising concerns worldwide about the contamination of food, feed, and environment with the residues of such chemical substances. High-performance liquid chromatography (LC) (HPLC) separates and quantifies the component compounds in a target sample matrix. It follows all the basic steps of LC by an automated approach. Being the center of the liquid chromatographic process, column is the site where all the chemistries among stationary phase, mobile phase, and target analytes take place. The chromatographic method performance primarily depends upon the HPLC column and is indicated or expressed in terms of separation/resolution, peak width, retention, and selectivity. The chromatographic strategy depends on the solvents used as mobile phase. It has become widely accepted that there are a limited number of mobile phase solvents suitable for RP-LC.
