ABSTRACT
Genomics and proteomics studies carried the promise of the identification of the full inventory of vesicular trafficking machinery components and regulator. As most proteins function in complexes, the future of this field also belongs to techniques such as fluorescence cross-correlation spectroscopy (FCCS) and forster resonance energy transfer (FRET) that enables identification and quantification of protein interactions in situ. Studies using these techniques should, in real time, provide a detailed picture of the molecular machines that mediate intracellular trafficking. This chapter focuses on imaging technologies such as correlative microscopy and live-cell imaging that has highlighted the plastic nature of the Golgi apparatus, an organelle that is sensitive to perturbation. Another approach that holds great potential for Golgi research and that overcomes the limitation of electron microscopy in acquiring temporal information is to combine light and electron microscopy through correlative light electron microscopy (CLEM).
