ABSTRACT
In open continuous culture, a continuous feed of medium flowing into a constant volume of growing culture and a constant withdrawal of an equal amount of culture produces a steady-state condition for growth. Continuous cultures could also render industrial exploitation of plant cell cultures more economical. The large scale propagation of various plants and glycosides by means of cultured plant cells demonstrates the relevance of the cell culture method to agriculture and industry. The establishment of open continuous cultures, in particular synchronous cultures greatly enhances possibilities for better understanding induction processes. The inoculum for continuous cultures generally is batch cultures of cell suspensions. The genetic stability of cell suspension cultures is of great concern. Semicontinuous culture has been mainly employed for maintaining stock cultures in an exponential growth phase over longer periods of time by avoiding nutrient limitation through frequent subculture.
