ABSTRACT
Eudragit RL is a nonbiodegradable copolymer of acrylic and methacrylic acid esters, which has been approved by regulatory agencies for oral delivery in polymeric form. This is in agreement with nanoparticles growth in the medical field, especially for drug delivery. The transcriptomic analysis was performed on Caco-2 cells following treatment by empty nanoparticles (NPs). This study is in addition to first viability and internalisation observations. NP-ERL were prepared using a double emulsion—the solvent evaporation method from three phases as described. Briefly, a primary water–oil emulsion was prepared by sonication of the internal aqueous phase and the organic phase. Flow cytometry assay were realized on samples for which cells were exposed to nanoparticles with or without serum in the culture medium. Several controls were realized and the results are congruent. A higher internalization rate is noticed when cells are exposed to nanoparticles in medium without serum.
