ABSTRACT
This chapter presents an overview of copolymer-phospholipid hybrid giant unilamellar vesicles (hGUVs). The molecular and macroscopic parameters necessary to obtain stable hybrid vesicles with different membrane structural levels (homogenous distribution of the components, nano-domain or micro-domain formation) will be first summarized, followed by a description of the different preparation methods used to obtain hGUVs. Finally, an overview of what is known about membrane properties of hGUVs will be proposed. Tips and advices will be given in the two last sections about the preparation protocols of hGUVs and techniques used to characterize their membrane properties.
