ABSTRACT
This chapter describes ultraviolet-visible (UV–Vis) spectroscopy because it is one of the most important analytical techniques used for a wide range of applications including food and beverage analysis, life sciences, clinical diagnostics, drug discovery, and environmental science. The part of the electromagnetic radiation employed by UV–Vis spectroscopy to interact with matter corresponds to the UV–Vis region. The components of spectrophotometer instruments for measuring sample transmittance as a function of the wavelength of electromagnetic radiation, with distinct configurations and options to display the results conveniently for the analyst. Those options are source of energy, device for isolating a narrow range of wavelengths, sample holder, detector for UV–Vis signal measurement, and signal output. The ideal light source would yield an adequate and constant intensity over the UV–Vis spectrum of interest with low noise and long-term stability, avoiding emitted radiation intensity fluctuation during the measurement.
