ABSTRACT

This chapter discusses conditions for Acetic Acid Bacteria (AAB) isolation and cultivation as well as how different preservation methods affect their cultivability. It reviews management of an AAB collection and analyzes the importance of strains quality controls, the role of databases and open platforms. Commonly different culture media need to be tested for isolating AAB from specific sources as the strains can have different requirements. For instance, a common medium is Glucose Yeast extract Carbonate, especially useful to isolate AAB from sources rich in sugar. Media containing acetic acid and ethanol, like Acetic Acid Ethanol and Reinforced Acetic acid Ethanol, are indicated for the high acetic acid-producing strains, as those isolated from spirit vinegars. The sub-culturing methods comprise periodical transplant of the cultures in a fresh medium in order to ensure growth and viability of cells. The cultures under mineral oil are stored in glass tubes located at controlled room temperatures.