ABSTRACT
Membrane chromatography is composed of a “filter-like” backbone, such as cellulose acetate, containing pore sizes larger than conventional chromatography resins. In flow-through chromatography operations, the product of interest is loaded onto the chromatography device under conditions in which the unwanted contaminants/impurities will bind to the ligands. Ion-exchange chromatography is characterized by using positively or negatively charged ligands to bind molecules within the mobile phase as they pass through the chromatography device. Affinity chromatography is defined by using ligands that have a specific affinity for specific moieties of particular molecules. Membrane chromatography operates under similar principles that define and characterize resin-based chromatography. Membrane chromatography is differentiated from conventional chromatography by the configuration of its stationary phase. Flow-through anion exchange chromatography is a powerful step that is commonly used for polishing applications. A smaller membrane chromatography device will require fewer buffers to flush residual product from the device.
