ABSTRACT
Locating the bloodstain in a crime scene is usually not a difficult task unless, as in some cases the stain may have changed color and thus becomes hard to identify. With the advent of innovative molecular biological techniques becoming the norm in the forensic laboratory, it is plausible to imagine the eventual replacement of the serological testing methods traditionally used to identify questioned stains with molecular biological techniques. In natural light examination of exhibits for brown or reddish-brown stains, powder or crystals of reddish-brown color, the areas should be demarcated. Spray the luminol directly onto the stain in question in the dark to see the luminescent reaction that can last for 15 seconds. A florence test is a very delicate test; a negative test may be obtained if choline content is low or stain is decomposed. A Barberio test depends upon presence of spermine in semen. Chemically, semen is a complex mixture of organic and inorganic compounds.
