ABSTRACT
This chapter provides isolation and screening processes that were carried out to get to the most efficient isolation of fungal in the production of enzyme. The isolation was diagnosed in the light of the diagnostic keys described by Klich and it turned out to be Aspergillus oryzae. The production of the enzyme was conducted by this isolation using solid state fermentation. The researchers also began to apply the immobilization technique on many enzymes, including the ß-galactosidase enzyme because of their important role in overcoming the inhibition occurring to the enzyme during decomposition of lactose and the accumulation of galactose. The ß-galactosidase is considered as one of the important enzymes in the dairy industry and resides in small intestines of the mammals. The enzyme ß-galactosidase produced from molds has higher purity than that produced using other organisms. Haider et al. indicated that the maximal activity of the purified enzyme from Aspergillus oryzae mold was at 50°C.
