ABSTRACT
Dhahran (DHA)-1 is an inducible plasmid-mediated AmpC beta-lactamase gene, which was reported as the most frequent cause of escalating morbidity and mortality rate among patients with infectious diseases. The aim of this study was to determine the prevalence of bla DHA-1 gene in Enterobacteriaceae clinical isolates in Indonesia. A total of 85 Enterobacteriaceae clinical isolates, including E. coli, Klebsiella. pneumoniae, E. cloacae, K. oxytoca, E. aerogenes, and Proteus mirabilis, were included in this study. The samples were collected from several private hospitals and clinical laboratories in Bandung, Indonesia, during a period between July 2014 and April 2015. The molecular detection of bla DHA-1 gene was carried out by PCR assay. We found that 32.9% of Enterobacteriaceae clinical isolates carried bla DHA-1 gene, which was predominantly found in K. pneumoniae (75%) followed by K. oxytoca (50%), E. cloacae (28.6%), and E.coli (14%). These results revealed a higher frequency of bla DHA-1 gene detected in Enterobacteriaceae clinical isolates in Indonesia compared with those in surveillance studies conducted worldwide. To our knowledge, this is the first molecular epidemiology report from Indonesia that showed the high prevalence of bla DHA-1 gene in Enterobacteriaceae clinical isolates.
Keywords: AmpC Beta-Lactamase; DHA-1; Enterobacteriaceae; PCR; Indonesia
