ABSTRACT
The main process for measuring spot intensity includes three stages [3]. In the first stage, called gridding, the position of each spot is assigned; compartments of the image containing one individual spot and background are therefore defined. In the second stage, called spot segmentation, the contour of each spot is determined. In the third stage, called intensity extraction (quantification), the intensity of each spot is calculated. Gene expression data analysis is the subsequent step of microarray analysis and is accomplished with different statistical and/or unsupervised learning methods. Robust segmentation is significant for accurate classification of gene expression and to extrapolate an assortment of spot quality measures. From the aforementioned stages, spot segmentation is the most challenging task.
